6 ^{Upstream processing for}

viral vaccines-Process

intensification

Sven Göbel and Lars Pelz

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany

Udo Reichl

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany and

Chair for Bioprocess Engineering, Otto-von-Guericke-

University Magdeburg, Magdeburg, Germany

Yvonne Genzel

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany

CONTENTS

6.1 Introduction...................................................................................................138

6.2 Motivation for Process Intensification.........................................................139

6.3 Parameters for Process Comparison and Evaluation ..................................140

6.4 From Batch Cultures to Intensified Processes.............................................143

6.5 Critical Factors for Virus Production at High Cell Density.......................146

6.6 Cell Retention Devices.................................................................................149

6.6.1 Spin Filters .......................................................................................152

6.6.2 Tangential Flow Filtration ...............................................................153

6.6.3 Alternating Tangential Flow............................................................155

6.6.4 Disc Centrifuge.................................................................................157

6.6.5 Inclined Settler .................................................................................159

6.6.6 Hydrocyclone....................................................................................160

6.6.7 Acoustic settler.................................................................................162

6.7 Use of Disposables.......................................................................................163

6.8 Conclusion....................................................................................................166

References..............................................................................................................167

DOI: 10.1201/9781003229797-6

137